human cc cell line hela  (ATCC)

Journal: International Journal of Women's Health

Article Title: Prediction of Cervical Cancer Progression Leveraging HPV16 Integration-Related Genes

doi: 10.2147/IJWH.S543345

Figure Lengend Snippet: NRF2 signaling inhibition sensitizes CC cells to cisplatin treatment. ( A ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. The data are representative of 3 biological replicates. ( B ) IC50 values of HeLa, SiHa and C33A cells treated with cisplatin for 48 h. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure, with ML385 maintained throughout the entire treatment period. The data are representative of 3 biological replicates. ( C ) RT-qPCR showing the mRNA expression of NQO1 in CC cell lines. Cells were pretreated with ML385 (1 µM) for 2 hours prior to cisplatin exposure. The data are representative of 3 biological replicates. ( D ) Representative histograms and gMFI of DCFH-DA in SiHa and HeLa cells. The data are representative of 3 biological replicates. *p< 0.05, **p< 0.01, ***p< 0.001.

Article Snippet: Human CC cell line HeLa, SiHa and C33A were obtained from the American Type Culture Collection (ATCC).

Techniques: Inhibition, Quantitative RT-PCR, Expressing

Journal: Discover Oncology

Article Title: The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells

doi: 10.1007/s12672-025-02456-9

Figure Lengend Snippet: Matrix stiffness promotes migration and invasion of CC cells and activates Piezo1. A Microscopy showing typical morphologies of HeLa and SiHa cells cultured on soft and stiff substrates. Scale bar: 100 μm; B Representative immunofluorescence images of HeLa and SiHa cells cultured on soft and stiff substrates in detecting phalloidin (green) and DAPI(blue). Scale bar: 50 μm; C Western blot results showed the expression of Piezo1 in HeLa and SiHa cells cultured on soft and stiff substrates. GAPDH was used as a control; D Quantization of C ( E , G ) Cell migration and invasion assays were performed using HeLa and SiHa cells cultured on soft and stiff substrates. F , H Quantization of E and G ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

Article Snippet: Human CC cell lines HeLa and SiHa were obtained from the American Type Culture Collection (ATCC) and were cultured at 37 °C in a humidified atmosphere of 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS with 100 U/ml penicillin and 100 U/ml streptomycin.

Techniques: Migration, Microscopy, Cell Culture, Immunofluorescence, Western Blot, Expressing, Control

Journal: Discover Oncology

Article Title: The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells

doi: 10.1007/s12672-025-02456-9

Figure Lengend Snippet: Knocking down of Piezo1 suppressed the migration and invasion of CC cells. A – C Microscopy imaging showed that the transfection was successful; D – F Western blot analysis of Piezo1 expression in HeLa and SiHa cells after Piezo1 knockdown; G , H Cell migration detected via wound healing assay after Piezo1 knockdown; I , J Quantization of G and H ; K , L Cell migration and invasion assays were examined by Transwell after Piezo1 knockdown; M , N Quantization of K and L ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

Article Snippet: Human CC cell lines HeLa and SiHa were obtained from the American Type Culture Collection (ATCC) and were cultured at 37 °C in a humidified atmosphere of 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS with 100 U/ml penicillin and 100 U/ml streptomycin.

Techniques: Migration, Microscopy, Imaging, Transfection, Western Blot, Expressing, Knockdown, Wound Healing Assay

Journal: Discover Oncology

Article Title: The increased matrix stiffness caused by LOXL2 activates Piezo1 channels to promote the migration and invasion of cervical cancer cells

doi: 10.1007/s12672-025-02456-9

Figure Lengend Snippet: ECM stiffness modulated CC progression through Piezo1. A , B Cell migration and invasion assays were performed using HeLa cells cultured on soft and stiff substrates after Piezo1 knockdown; C , D Quantization of A and B ; E , F Cell migration and invasion assays were performed using SiHa cells cultured on soft and stiff substrates after Piezo1 knockdown; G , H Quantization of E and F ; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001

Article Snippet: Human CC cell lines HeLa and SiHa were obtained from the American Type Culture Collection (ATCC) and were cultured at 37 °C in a humidified atmosphere of 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FBS with 100 U/ml penicillin and 100 U/ml streptomycin.

Techniques: Migration, Cell Culture, Knockdown